Tom Brown Group

Squaramides and Ureas: A Flexible Approach to Polymerase-Compatible Nucleic Acid Assembly

Arun Shivalingam, Lapatrada Taemaitree, Afaf H. El-Sagheer, Tom Brown. Angew. Chem. Int. Ed. 59 (28), 11416-11422, 2020.

2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis

Jack S Hardwick, Marius M Haugland, Afaf H El-Sagheer, Denis Ptchelkine, Frank R Beierlein, Andrew N Lane, Tom Brown, Janet E Lovett, Edward A Anderson. Nucleic Acids Res. 48 (6), 2830-2840, 2020.

Lighting Up DNA with the Environment-Sensitive Bright Adenine Analogue qAN4

Anders F. Füchtbauer, Moa S. Wranne, Sangamesh Sarangamath, Mattias Bood, Afaf H. El-Sagheer, Tom Brown, Henrik Gradén, Morten Grøtli, L. Marcus Wilhelmsson. ChemPlusChem 85 (2), 319-326, 2020.

DNA Structural Changes Induced by Intermolecular Triple Helix Formation

Ibrahim Sayoh, David A. Rusling, Tom Brown, Keith R. Fox*. ACS Omega 5 (3), 1679-1687, 2020.

Searching for the ideal triazole: Investigating the 1,5-triazole as a charge neutral DNA backbone mimic

Ysobel R. Baker, Diallo Traoré, Przemyslaw Wanat, Agnès Tyburn, Afaf H. El-Sagheer, TomBrown. Tetrahedron 76 (7), 130914, 2020.

Oxidative DNA Cleavage with Clip-Phenanthroline Triplex-Forming Oligonucleotide Hybrids

Alessandro Panattoni, Afaf H. El-Sagheer, Tom Brown, Andrew Kellett, Michal Hocek. ChemBioChem 21, 991-1000, 2020.

Stability of the different arms of a DNA tetrahedron and its interaction with a minor groove ligand

Michael L.J. Carter, David A. Rusling, Steven Gurr, Tom Brown, Keith R. Fox. Biophysical Chemistry 25, 106270, 2020.

Optimised oligonucleotide substrates to assay XPF ERCC1 nuclease activity for the discovery of DNA repair inhibitors

A. M. Thomas, S. Brolih, J. F. McGouran, A. H. El-Sagheer, D. Ptchelkine, M. Jones P. J. McHugh and T. Brown. Chem. Commun. 55 (78), 11671-11674, 2019.

A hydroxamic-acid-containing nucleoside inhibits DNA repair nuclease SNM1A

William Doherty, Eva-Maria Dürr, Hannah T. Baddock, Sook Y. Lee, Peter J. McHugh, Tom Brown, Mathias O. Senge, Eoin M. Scanlana, Joanna F. McGouran. Organic and Biomolecular Chemistry 17, 8094-8105, 2019.

Radiolabeled oligonucleotides targeting the RNA subunit of telomerase inhibit telomerase and induce DNA damage in telomerase-positive cancer cells

M. R. Jackson, B. M. Bavelaar, P. A. Waghorn, M. R. Gill, A. H. El-Sagheer, T. Brown, M. Tarsounas and K. A. Vallis. Cancer Res. 2019.

Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting

P. Klimkowski, S. De Ornellas, D. Singleton, A. H. El-Sagheer and T. Brown. Org. Biomol. Chem. 17, 5943-5950, 2019.

Curtailing their negativity

A. Brown and T. Brown. Nat. Chem. 11 (6), 501-503, 2019.

Synthesis and biophysical properties of carbamate-locked nucleic acid (LNA) oligonucleotides with potential antisense applications

C. Thorpe, S. Epple, B. Woods, A. H. El-Sagheer and T. Brown. Org. Biomol. Chem. 17, 5341-5348, 2019.

Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices

Angela F. De Fazio, Afaf H. El-Sagheer, Jason S. Kahn, Iris Nandhakumar, Matthew Richard Burton, Tom Brown, Otto L. Muskens, Oleg Gang*, and Antonios G. Kanaras*. ACS Nano 13 (5), 5771-5777, 2019.

“Split-and-Click” sgRNA

Lapatrada Taemaitree, Arun Shivalingam, Afaf H. El-Sagheer, Tom Brown. Methods in molecular biology 2162, 61-78, 2019.

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

L. Taemaitree, A. Shivalingam, A. H. El-Sagheer and T. Brown. Nat. Commun. 10, 2019.

DNA-Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals

M. Moros, M. Kyriazi, A. H. El-Sagheer, T. Brown, C. Tortiglione and A. G. Kanaras. ACS Appl. Mater. Interfaces 11 (15), 13905-13911, 2018.

Squaramide-Based 5'-Phosphate Replacements Bind to the DNA Repair Exonuclease SNM1A

E. Dürr, W. Doherty, S. Y. Lee, A. H. El-Sagheer, A. Shivalingam, P. J. McHugh, T. Brown and J. F. McGouran. ChemistrySelect 3 (45), 12824-12829, 2018.

Synthesis, oligonucleotide incorporation and fluorescence properties in DNA of a bicyclic thymine analogue

C. P. Lawson, A. F. Fuchtbauer, M. S. Wranne, T. Giraud, T. Floyd, B. Dumat, N. K. Andersen, A. H. El-Sagheer, T. Brown, H. Graden, L. M. Wilhelmsson and M. Grotli. Sci. Rep. 8, 2018.

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation

J. Chen, Y. R. Baker, A. Brown, A. H. El-Sagheer and T. Brown. Chem. Sci. 9, 8110-8120, 2018.